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Amino Acids and Peptides
面议Proteins
面议Nucleic Acid Components
面议Catecholamines/Catecholamine Metabolism Acids
面议Cholesterolesters
面议Chiralic Analysis
面议Analysis of Pharmaceuticals with UltraSep ES PHARM
面议Analysis of Pharmaceuticals with UltraSep ES MLD
面议Selectivity Pack TRIS
面议Other Special Columns
面议
Anion Exchangers | |||
UltraSep ES SAX W (Anorganic ions according to DIN 38405) | |||
These anion exchangers are so called „permanently loaded“ ion exchangers. They exhibit the excellent performance of silicas and the good stability of RP-phases, which leads to low costs. | |||
Stability of UltraSep ES SAXW with phthalic acid/TRIS buffer (1.25/2.5 mM) at pH 6.4 | |||
Permanently loaded ion exchangers are not only useful to separate inorganic ions (see Application ANION1) but also to separate natural products with aqueous buffers (nucleotides). They cannot be used with organic-aqueous eluents. Our permanently loaded ion exchangers are easily to regenerate. For this, we offer a special solution or a regeneration in our firm. If there is a need to chromatograph aqueous solutions highly contaminated with organic material, we recommend to use additionally a RP precolumn (length 33 mm) in front of the precolumn cartridge ( ANION2 Tap water from Berlin). By lowering of the strength of the eluent, phosphate appears between nitrite and bromide without the necessity to increase the pH noticeably In this way, there are good conditions for the conductivity detection too. Furthermore, fluoride is well separated from the injection peak (see Application ANION3). | |||
UltraSep ES SAX W Quick Columns | ||
See Applications ANION5 and ANION6 | ||
UltraSep ES SAX W33 (Determination of ascorbic acid) | ||
See Applications ANION9 | ||
UltraSep ES SAX NN (Rapid determination of nitrite, nitrate, and bromide in drinking water and foodstuffs) | ||
By this exchanger concentrations of nitrite and nitrate can be determined considerably faster and more sensitive than with customary methods. Nitrate, which can be determined by the photometric method only relatively insensitively, is determined by chromatography at 220 nm without previous enrichment to 0.1 mg/l. Bromide is determined at 210 nm with high sensitivity. Iodide can be determined also directly at 225 nm. For a quick determination of iodide you can use a column of UltraSep ES SAX NN with a length of 33 mm. The selectivity and rapidity of the determination (phosphoric acid as eluent) can be changFor columns with endcapped packings an additional charge of 10.00 € is calculated. ed by the pH value (at best pH 3 – 5). Furthermore, the velocity of elution can also be varied by the concentration of phosphoric acid (at best 10 – 30 mM; see Application ANION4). | ||
UltraSep ES RP NN (Determination of nitrite, nitrate, bromate and bromide in drinking water and foodstuffs) | ||
See Applications ANION7 and ANION8 |
| Examples of Analyzed Compounds | Analyzed in Application | Go to |
Ascorbic acid | AN9 | ANION1 | |
| Bromate | AN7, AN8 | ANION2 |
Bromide | AN1, AN3, AN4, AN5, AN6, AN7 | ANION3 | |
| Carbonate | AN2 | ANION4 |
| Chloride | AN1, AN2, AN3, AN5, AN6 | ANION5 |
| Fluoride | AN3 | ANION6 |
| Nitrate | AN1, AN3, AN4, AN5, AN6, A7 | ANION7 |
| Nitrite | AN1, AN3, AN4, AN5, AN6, A7 | ANION8 |
| Phosphate | AN3, AN6 | ANION9 |
| Sulfate | AN1, AN2, AN3, AN5, AN6 | |
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COLUMNS and PRICES (€) | ||||
Column size (mm) | ||||
150 x 3 | 125 x 3 | 60 x 3 | 33 x 3 | |
UltraSep ES SAX W | 270.00 | |||
UltraSep ES SAX W Guard column RP 18 * | 125.00 | |||
UltraSep ES SAX W QC | 255.00 | 250.00 | ||
UltraSep ES SAX QCM | 270.00 | |||
UltraSep ES SAX NN | 265.00 | 245.00 | ||
UltraSep ES RP NN | 240,00 | 240,00 | ||
UltraSep ES SAX W33 | 295,00 | |||
* Recommended as precolumn for samples with high content of organic substances. |
Size (mm) | |||
10 x 3 | 5 x 3 *** | |||
Precolumn cartridges (Price per pack = 5 pieces) | 170.00 | 170.00 | ||
Activator for ES SAX W (1 ml) | 12.00 | |||
Regeneration solution (30 ml) for regeneration by customer | 33.00 | |||
*** Only for UltraSep ES SAX W QC. |