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● 高效的装柱系统
● 可受压5000psig,适用于SFC超临界流体色谱柱
● 用MultiPacker 装柱机可轻松装柱,轴向SFC柱是基于轴向弹簧压缩柱开发设计的。SFC柱可以在相同的MultiPacker 装柱系统上轻松装填, 但设计时考虑了SFC的要求。 密封系统也按特殊工艺生产的,其zui大可耐压达5000psig。虽然也采用轴向加压的方式装柱,但由于SFC系统典型的高备压以及流动相的低粘度特性,柱头也不太可能出现塌陷,所以在SFC柱中不使用弹簧。
?轴向SFC 柱硬件规格 | |
内表面: | 电抛光型316不锈钢,PTFE |
柱身: | 电抛光型316不锈钢 |
活塞: | 电抛光型316不锈钢 |
螺纹锁定杆: | 6061铝 |
柱底板: | 抛光型316不锈钢 |
螺栓零件: | 8级,适合压力容器用 |
筛板: | 钝化烧结的316不锈钢 |
密封圈: | PTFE |
唇形密封: | Turcon |
受力环: | 铜 |
压力耐压: | 5000psig (345bar) |
zui大耐热: | 135 °C (275 °F) |
SFC密封系统
轴向SFC柱的密封材料可以和SFC系统的超临界二氧化碳相兼容。密封系统可以在高达5000psig(345bar)压力下连续运行。
活塞
一个PTFE材质的O形主密封圈和一个加弹簧的Turcon 次密封圈保证了活塞的*密封。
柱出口
柱出口密封系统是由一个加弹簧的Turcon 密封圈和铜受力环共同构成。
SFC柱硬件套装
如下所示,用户可以用一个订单号订购标准的25mm和50mm内径的带水浴套SFC柱硬件套装,以下两页列出了每个套装的零件
Axial SFC Columns
包括 | |||
柱尺寸 | 货号 | 描述 | 货号 |
25mm i.d. | |||
25mm i.d. x 40cm, Water-Jacketed | AY2002-W25040 | Base Column Parts Kit*, 40cm long column, for bed lengths up to 15cm | SY2002-W25040 |
AY2002-025A01 | Piston Assembly with 1μm frit | ||
AF0000-C02501 | Outlet Frit, 1μm | ||
AS2002-025A07 | 1/16”o.d., 0.020”i.d. Connecting Tube and Fittings | ||
25mm i.d. x 70cm, Water-Jacketed | AY2002-W25070 | Base Column Parts Kit*, 70cm Iong column, for bed Iengths up to 30cm | SY2002-W25070 |
AY2002-025A01 | Piston Assembly with 1μm frit | ||
AF0000-C02501 | Outlet Frit, 1μm | ||
AS2002-025A07 | 1/16”o.d., 0.020”i.d. Connecting Tube and Fittings | ||
50mm i.d. | |||
50mm i.d. x 40cm, Water-Jacketed | AY2002-W50040 | Base Column Parts Kit*, 40cm long column, for bed lengths up to 15cm | SY2002-W50040 |
AY2002-050A01 | Piston Assembly with 1μm frit | ||
AF0000-B05001 | Outlet Frit, 1μm | ||
AS2002-050A07 | 1/8”o.d., 0.060”i.d. Connecting Tube and Fittings | ||
50mm i.d. x 70cm, Water-Jacketed | AY2002-W50070 | Base Column Parts Kit*, 70cm Iong column, for bed Iengths up to 30cm | SY2002-W50070 |
AY2002-050A01 | Piston Assembly with 1μm frit | ||
AF0000-B05001 | Outlet Frit, 1μm | ||
AS2002-050A07 | 1/8”o.d., 0.060”i.d. Connecting Tube and Fittings |
超临界流体色谱柱(SFC)
1879年*次发现了超临界流体,二十世纪五十年代人们就利用超临界流体进行萃取应用。到二十世纪八十年代越来越多的人把超临界流体作为流动相应用到SFC中。SFC中zui常见的流动相是二氧化碳,因为超临界的二氧化碳对很多物质有很好的溶解度和很大的上样量。此外,超临界二氧化碳和其它超临界流体的粘度很低,这使得快速分离和萃取成为可能。超临界流体的易挥发性使得收集的馏分可以在自然条件下的轻松浓缩。
超临界流体色谱柱今天已成为另一种高效液相色谱,亦或正相亦或反相,它是分析热不稳定中小分子的理想工具。任何可溶于甲醇或更弱极性溶剂的分子都可以用SFC分析,很多很难用其它技术分离的强碱可以在SFC上快速高效的分离并有很好的峰形。因为其分离的高速度以及馏分收集的简单易行,SFC技术近来在药物研发和药物纯化领域的应用也越来越多。SFC要求柱硬件和密封装置在高压下不泄漏。格雷斯公司的轴向SFC柱正是基于这种要求而设计的,此外采用已被广泛接受的轴向加压技术的装填技术可以得到更长的柱使用寿命。
如何订购轴向SFC柱
轴向SFC柱可以订购一整套的柱硬件,见宣传资料74页,用户也以根据要求自己选购,如下所述。格雷斯公司目前提供两种规格的轴向SFC柱,40cm和70cm长度的两种。40 cm长的柱管可以zui长装填15cm柱长的色谱柱,70cm长的柱管可以zui长装填30cm柱长的色谱柱。
超临界流体色谱柱完整的柱硬件有四个部件组成:
1. SpringTM弹簧柱基本套装包括:
a. 轴向SFC柱管
b. 密封圈和O形环
c. 锁定装置
d. 柱尾法兰
e. 组装零件
f. 装柱垫块(70cm长柱管用)
2. 活塞套装包括活塞、筛板、PTFE热缩膜和O形环,可以订购带1μm或5μm筛板孔径的活塞套装。
3. 出口筛板。可选择1μm或5μm筛板。
4. 管路连接。选择您需要的内外径的管路
有经验的轴向SFC柱用户可以根据需要选择零件自己来组装完整的轴向SFC柱。
Axial SFC Columns
包括 | ||
柱尺寸 | 描述 | 货号 |
25mm i.d. | ||
1. Base Parts Kit | Non-Water-Jacketed Axial SFC Column Kit, 40cm long, for bed lengths up to 15cm | AY2002-025040 |
Water-Jacketed Axial SFC Column Kit, 40cm long, for bed lengths up to 15cm | AY2002-W25040 | |
Non-Water-Jacketed Axial SFC Column Kit, 70cm long, for bed lengths up to 30cm | AY2002-025070 | |
Water-Jacketed AxiaI SFC Column Kit, 70cm Iong, for bed Iengths up to 30cm | AY2002-W25070 | |
2. Piston Assembly | Piston Assembly with 1μm frit | AY2002-025A01 |
Piston Assembly with 5μm frit | AY2002-025A02 | |
3. Outlet Frit | Outlet Frit, 1μm | AF0000-C02501 |
Outlet Frit, 5μm | AF0000-C02503 | |
4. Tubing and Connectors | 1/16”o.d., 0.020”i.d. Connecting Tube and Fittings | AF2002-025A06 |
1/16”o.d., 0.040”i.d. Connecting Tube and Fittings | AF2002-025A07 | |
50mm i.d. | ||
1. Base Parts Kit | Non-Water-Jacketed Axial SFC Column Kit, 40cm long, for bed lengths up to 15cm | AY2002-050040 |
Water-Jacketed AxiaI SFC Column Kit, 40cm Iong, for bed Iengths up to 15cm | AY2002-W50040 | |
Non-Water-Jacketed AxiaI SFC Column Kit, 70cm Iong, for bed Iengths up to 30cm | AY2002-050070 | |
Water-Jacketed AxiaI SFC Column Kit, 70cm Iong, for bed Iengths up to 30cm | AY2002-50070 | |
2. Piston Assembly | Piston Assembly with 1μm frit | AY2002-050A01 |
Piston Assembly with 5μm frit | AY2002-050A02 | |
3. Outlet Frit | Outlet Frit, 1μm | AF0000-B05001 |
Outlet Frit, 5μm | AF0000-B05003 | |
4. Tubing and Connectors | 1/16”o.d., 0.040”i.d. Connecting Tube and Fittings | AF2002-050A07 |
1/8”o.d., 0.060”i.d. Connecting Tube and Fittings | AF2002-050A08 | |
1/8”o.d., 0.085”i.d. Connecting Tube and Fittings | AF2002-050A09 |
上述套装中的独立零件和零件组合清单,请见后面以及本页
SFC柱重装套装
每个重装套装中含有可重装一次的易耗件。
Repack Kit for SpringTM Columns
包括 | |||
柱尺寸 | 货号 | 描述 | 货号 |
25mm i.d. | |||
With 1pm Frits | AF0000-C02501 | Outlet Frit, 1μm | AY2002-025A14 |
AH0000-R025A2 | SeaI Ring Set for AxiaI SFC Column Outlet | ||
AF0000-D02501 | Outlet Disperser Frit | ||
AY2002-025A12 | Piston Frit Replacement Kit*, 1μm | ||
With 5pm Frits | AF0000-C02503 | Outlet Frit, 5μm | AY2002-025A15 |
AF0000-D02501 | Outlet Disperser Frit | ||
AH0000-R025A2 | SeaI Ring Set for AxiaI SFC Column Outlet | ||
AY2002-025A13 | Piston Frit Replacement Kit*, 5μm | ||
50mm i.d. | |||
With 1pm Frits | AF0000-B05001 | Outlet Frit, 1μm | AY2002-050A12 |
AH0000-R050A2 | Seal Ring Set for AxiaI SFC Column Outlet | ||
AF0000-D05001 | Outlet Disperser Frit | ||
AY2002-050A14 | Piston Frit Replacement Kit*, 1μm | ||
With 5pm Frits | AF0000-B05003 | Outlet Frit, 5μm | AY2002-025A15 |
AF0000-D05001 | Outlet Disperser Frit | ||
AH0000-R050A2 | Seal Ring Set for AxiaI SFC Column Outlet | ||
AY2002-050A15 | Piston Frit Replacement Kit*, 5μm |
SFC柱可更换的零件
除了上述的SFC柱重装套装外,您还可以在下面的清单中订购每一个零件
SpringTM Column Replacement Parts
描述 | For 25mm i.d. | For 50mm i.d. |
0utlet Disperser Frit | AF0000-D02501 | AF0000-D05001 |
Outlet Frit, 1μm | AF0000-C02501 | AF0000-B05001 |
Outlet Frit, 5μm | AF0000-C02503 | AF0000-B05003 |
Seal Ring Set for Axial SFC Column Outlet | AH0000-R025A2 | AH0000-R050A2 |
Replacement Bolts, Nuts, and Washers for Non-Water-Jacketed Column | AY2002-025A08 | AY2002-050A10 |
Replacement Botls, Nuts, and Washers for Water-Jacketed Column | AY2002-025A11 | AY2002-050A13 |
Threaded Spacer for 40cm Long Column | AS2002-025000 | AS2002-050000 |
Threaded Spacer for 70cm Long Column | AS2002-025001 | AS2002-050001 |
Threaded Flange | AS2002-025002 | AS2002-050002 |
Packing Spacer | AS2002-025003 | AS2002-050003 |
Unpacking Spacer for 70cm Long Columns | AS2002-025005 | AS2002-050005 |
Piston | AY2002-025007 | AY2002-050007 |
Piston Frit Replacement Kit, 1μm;Piston O-ring, spring-loaded seal, PTFE sleeve, 1μm Double-Density Frit | AY2002-025A12 | AY2002-050A14 |
Piston Frit Replacement Kit, 5μm;Piston O-ring, spring-loaded seal, PTFE sleeve, 5μm Double-Density Frit | AY2002-025A13 | AY2002-050A15 |
Piston SeaI for Axial SFC Column | AY2002-025011 | AY2002-050011 |
PTFE Sleeve | AS2002-025010 | AS2002-050010 |