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Spring弹簧柱/制备柱简介
用于制备和工业纯化的静态轴向加压柱
高效,耐用,经济
MODcol®SPRINGTM弹簧柱/制备柱能自动补充制备柱使用过程中柱床下沉形成的空隙,因此能延长柱寿命和提高总体柱性能和经济效益。
这项令人兴奋的新技术利用弹簧的收缩原理来保持柱床的轴向加压,而不用传统的水压系统。柱子里的弹簧稳定地保持压力以解决不可避免的填料损坏问题。随着柱床的逐渐收缩，弹簧伸展，弥补死体积并防止空隙的产生，这样就得到了优异的柱效并延长了使用时间。
Spring弹簧柱/制备柱 使用说明
简单方便，不象其它的轴向加压柱， MODcol SPRING柱是独立自足的，一旦完成装柱，可以脱离装柱机使用，它能保持传统轴向加压技术同样的性能和优点，却大大减少了费用。另外， 自己自足的设计可保证‘错误允许’操作。这意味着您可以在任何实验室有zui大的灵活性，例如空间的限制，即使在断电的情况下也同样保持柱效。
灵活通用
MODcol®SPRINGTM可以根据您的需要应用于各种色谱填料。所有的 5um 或者5um以上的传统填料，包括硅胶基的，聚合物的甚至软胶，都可以达到优异的柱效和更长的柱寿命。另外，配合高压活塞，SpringTM柱可用于SFC（超临界流体色谱）。
对于需精确控制温度的应用，SPRINGTM柱可提供整体的水浴夹套。
弹簧柱规格
可选择预装柱或自装柱
SPRINGTM柱有25mm, 50mm, 和100mm三种直径和多种柱长，以VYDAC® 填料装填的预装柱。另外，MODcol空柱和MODcol MULTIPACER®装柱机能提供*灵活的自己装柱和维护
弹簧柱/制备柱
● 持续不断的轴向压缩防止塌陷形成从而确保色谱柱的长寿命
● 和普通HPLC柱一样可以在任何地方使用
● 轴向压缩条件下装填获得高柱效
弹簧柱填示意图
弹簧柱硬件
SpringTM弹簧柱需和MultiPacker柱装填机配套使用，具有*的优点：
● 高效的轴向压缩
● 更长的填料使用寿命
● 便携式的轴向压缩柱
● 装柱简单方便
如何订购和MultiPacker装柱系统配套的SpringTM弹簧柱硬件弹簧柱可以整套订购，请咨询上海宸乔生物科技有限公司
用户根据要求自己选择其中的部件，相关客户定制件，请咨询上海宸乔生物科技有限公司。弹簧柱在长度上有两种规格40cm和70cm。40cm柱管zui长可装填15cm长的柱子。70cm柱管zui长可装填30cm长的柱子。
每个弹簧柱套装有五个部件组成。对于用户定制柱，下列的每个部件都需要订购。
1. 弹簧柱基本套装包括
a. 柱管
b. 密封圈和O形环
c. 锁定装置
d. 导管
e. 法兰
f. 组装螺栓零件
g. 柱长垫块(70cm柱用)－未附照片部
2. 弹簧柱活塞套装有活塞、筛板、PTFE热缩膜和O形环，可以分别订购1um、2um、5um筛板孔径的活塞套装。
3.弹簧柱套装。可以根据您的要求选购不同压力范围的弹簧套装。为保持轴向压缩力，弹簧的弹力必须要超过实际的操作压力。
4. 出口筛板。选购1um、2um、5um孔径的筛板。对于101 mm内径的柱子，出口筛板和活塞套装是*一样的。
5. 管路连接。选购适合您需要的内外径的管路
下面的指导将会帮助您选择适合您需要的部件组合。如仍需帮助请上海宸乔生物科技有限公司的。
弹簧柱硬件套装：
我们提供成套的25mm、50mm、101mm内径的标准SpringTM弹簧柱硬件套装,如下表所示，用一个产品号就可以订购。每个套装都配2um的筛板和1000－2000psig的弹簧。如客户有不同的需求，可以从后面选择部件自由组合定制的弹簧柱/制备柱。
后面详细列出了每个部件和替换零件。
硬件套装 ：
| 包括 | |||
| 柱尺寸 | 货号 | 描述 | 货号 |
| 25mm i.d. | |||
| 25mm i.d. x 40cm, Non-Water-Jacketed | SA2002-025040 | Base Column Parts Kit*, 40cm long column, for bed lengths up to 15cm | SC2002-025040 |
| AS2002-025A09 | Piston Assembly with 2μm frit | ||
| AF0000-C02502 | Outlet Frit, 2μm | ||
| AS2002-025A03 | Spring Kit for packing pressures from 1000 to 2000psig | ||
| AS2002-025A07 | 1/16”o.d., 0.040”i.d. Connecting Tube and Fittings | ||
| 25mm i.d. x 70cm, Non-Water-Jacketed | SA2002-025070 | Base Column Parts Kit*, 70cm long column, for bed lengths up to 30cm | SC2002-025070 |
| AS2002-025A09 | Piston Assembly with 2μm frit | ||
| AF0000-C02502 | Outlet Frit, 2μm | ||
| AS2002-025A03 | Spring Kit for packing pressures from 1000 to 2000psig | ||
| AS2002-025A07 | 1/16”o.d., 0.040”i.d. Connecting Tube and Fittings | ||
| 50mm i.d. | |||
| 50mm i.d. x 40cm, Non-Water-Jacketed** | SA2002-050040 | Base Column Parts Kit*, 40cm long column, for bed Iengths up to 15cm | SC2002-050040 |
| AS2002-050A01 | Piston Assembly with 2μm frit | ||
| AF0000-B05002 | Outlet Frit, 2μm | ||
| AS2002-050A03 | Spring Kit for packing pressures from 1000 to 2000psig | ||
| AS2002-050A07 | 1/6”o.d., 0.040”i.d. Connecting Tube and Fittings | ||
| AS2002-050A09 | 1/8”o.d., 0.060”i.d. Connecting Tube and Fittings | ||
| 50mm i.d. x 70cm, Non-Water-Jacketed** | SA2002-050070 | Base Column Parts Kit*, 70cm Jong column, for bed Iengths up to 30cm | SC2002-050070 |
| AS2002-050A01 | Piston Assembly with 2μm frit | ||
| AF0000-B05002 | Outlet Frit, 2μm | ||
| AS2002-050A03 | Spring Kit for packing pressures from 1000 to 2000psig | ||
| AS2002-050A07 | 1/16”o.d., 0.040”i.d. Connecting Tube and Fittings | ||
| AS2002-050A09 | 1/8”o.d., 0.060”i.d. Connecting Tube and Fittings | ||
| 101mm i.d. | |||
| 101mm i.d. x 40cm, Water-Jacketed | SA4004-101040 | Base Column Parts Kit*, 40cm long column, for bed lengths up to 15cm | SC4004-101040 |
| ?2 x AS4004-101A06 | Piston Assembly with 2μm frit | ||
| AS4004-101A03 | ?Spring Kit for packing pressures up to 2000psig | ||
| AS2002-050A08 | 1/8”o.d., 0.060”i.d. Connecting Tube and Fittings | ||
| 101mm i.d. x 70cm, Water-Jacketed | SA4004-101070 | Base Column Parts Kit*, 70cm long column, for bed lengths up to 30cm | SC4004-101070 |
| 2 x AS4004-101A06 | Piston Assembly with 2μm frit | ||
| AS4004-101A03 | Spring Kit for packing pressures up to 2000psig | ||
| AS2002-050A08s | 1/8”o.d., 0.060”i.d. Connecting Tube and Fittings | ||
硬件
有经验的弹簧柱用户可以根据自己的要求定制弹簧柱零件，组装一个完整的弹簧柱需要五个零件。
弹簧柱零件：
| 柱尺寸 | 描述 | 货号 |
| 25mm i.d. | ||
| 1. Base Parts Kit | Non-Water-Jacketed Kit, 40cm long, for bed Iengths up to 15cm | SA2002-025040 |
| Water-Jacketed Kit, 40cm Iong, for bed Iengths up to 15cm | SA2002-W25040 | |
| Non-Water-Jacketed Kit, 70cm Iong, for bed lengths up to 30cm | SA2002-025070 | |
| Water-Jacketed Kit, 70cm Iong,for bed Iengths up to 30cm | SA2002-W25070 | |
| 2. Piston Assembly | Piston Assembly with 1μm frit | AS2002-025A01 |
| Piston Assembly with 2μm frit | AS2002-025A09 | |
| Piston Assembly with 5μm frit | AS2002-025A02 | |
| 3. Spring Kit | Spring Kit for packing pressures from 1000 to 2000psig | AS2002-025A03 |
| Spring Kit for packing pressures from 500 to 900psig | AS2002-025A04 | |
| Spring Kit for packing pressures up to 400psig | AS2002-025A05 | |
| 4. Outlet Frit | Outlet Frit, 1μm | AF0000-C02501 |
| Outlet Frit, 2μm | AF0000-C02502 | |
| 0utlet Frit, 5μm | AF0000-C02503 | |
| 5. Tubing and Connectors | 1/16”o.d., 0.020”i.d. Connecting Tube and Fittings | AS2002-025A06 |
| 1/16”o.d., 0.040”i.d. Connecting Tube and Fittings | AS2002-025A07 | |
| 50mm i.d. | ||
| 1. Base Parts Kit | Non-Water-Jacketed Kit, 40cm Iong, for bed Iengths up to 15cm | SA2002-050040 |
| Water-Jacketed Kit, 40cm long．for bed Iengths up to 15cm | SA2002-W50040 | |
| Non-Water-Jacketed Kit, 70cm Iong,for bed lengths up to 30cm | SA2002-050070 | |
| Water-Jacketed Kit，70cm Iong,for bed Iengths up to 30cm | SA2002-W50070 | |
| 2. Piston Assembly | Piston Assembly with 1μm frit | AS2002-050A01 |
| Piston Assembly with 2μm frit | AS2002-050A11 | |
| Piston Assembly with 5μm frit | AS2002-050A02 | |
| 3. Spring Kit | Spring Kit for packing pressures from 1000 to 2000psig | AS2002-050A03 |
| Spring Kit for packing pressures from 500 to 900psig | AS2002-050A05 | |
| Spring Kit for packing pressures up to 400psig | AS2002-050A06 | |
| 4. Outlet Frit | Outlet Frit, 1μm | AF0000-B05001 |
| Outlet Frit, 2μm | AF0000-B05002 | |
| 0utlet Frit, 5μm | AF0000-B05003 | |
| 5. Tubing and Connectors | 1/16”o.d., 0.040”i.d. Connecting Tube and Fittings | AS2002-050A07 |
| 1/8”o.d., 0.060”i.d. Connecting Tube and Fittings | AS2002-050A08 | |
| 1/8”o.d., 0.085”i.d. Connecting Tube and Fittings | AS2002-050A09 | |
| 101mm i.d. | ||
| 1. Base Parts Kit | Non-Water-Jacketed Kit, 40cm Iong, for bed Iengths up to 15cm | SA4004-N10140 |
| Water-Jacketed Kit, 40cm long, for bed lengths up to 15cm | SA4004-101040 | |
| Non-Water-Jacketed Kit, 70cm Iong, for bed lengths up to 30cm | SA4004-N10170 | |
| Water-Jacketed Kit, 70cm Iong, for bed Iengths up to 30cm | SA4004-101070 | |
| 2. Piston Assembly (Need Qty．2) | Piston Assembly with 1μm frit | AS4004-101A01 |
| Piston Assembly with 2μm frit | AS4004-101A06 | |
| Piston Assembly with 5μm frit | AS4004-101A02 | |
| 3. Spring Kit | Spring Kit for packing pressures from 1000 to 2000psig | AS4004-101A03 |
| Spring Kit for packing pressures from 500 to 900psig | AS4004-101A04 | |
| Spring Kit for packing pressures up to 400psig | AS4004-101A05 | |
| 4. Tubing and Connectors | 1/8”o.d., 0.060”i.d. Connecting Tube and Fittings | AS2002-050A08 |
| 1/8”o.d., 0.085”i.d. Connecting Tube and Fittings | AS2002-050A09 | |
上述套装和组合里的每个零件在第后面表格中都一一列出了。
小贴士
请选择正确的筛板尺寸以确保没有填料颗粒跑出柱子而影响背压，请见下面的*。
| 颗粒大小 | *筛板孔隙 |
| <5um | 1um |
| 5-10um | 2um |
| >10um | 5um |
SpringTM弹簧柱重装套装
重装柱时，常见的2μm和5μm孔径的筛板是和对应的的套装打包提供的, 1μm孔径的筛板是可以从下面的重装零件表中订购。
25mm和50mm内径弹簧柱的重装套装可满足三次重装，101mm内径弹簧柱的重装套装可满足一次重装。
弹簧柱重装套装
| 包括 | ||||
| 柱尺寸 | 数量 | 货号 | 描述 | 货号 |
| 25mm i.d. | ||||
| With 2μm Frits | 3 | AF0000-C02502 | Outlet Frit, 2μm | AS2002-025A16 |
| 3 | AH0000-R025A1 | Outlet O-ring Set | ||
| 3 | AF0000-D02501 | Outlet Disperser Frit | ||
| 3 | AS2002-025A14 | Piston Frit Replacement Kit*, 2μm | ||
| With 5μm Frits | 3 | AF0000-C02503 | 0utlet Frit, 5μm | AS2002-025A17 |
| 3 | AH0000-R025A1 | Outlet O-ring Set | ||
| 3 | AF0000-D02501 | Outlet Disperser Frit | ||
| 3 | AS2002-025A13 | Piston Frit Replacement Kit*, 5μm | ||
| 50mm i.d. | ||||
| With 2μm Frits | 3 | AF0000-B05002 | Outlet Frit, 2μm | AS2002-050A18 |
| 3 | AH0000-R050A1 | Outlet O-ring Set | ||
| 3 | AF0000-D05001 | Outlet Disperser Frit | ||
| 3 | AS2002-050A16 | Piston Frit Replacement Kit*, 2μm | ||
| With 5μm Frits | 3 | AF0000-B05003 | 0utlet Frit, 5μm | AS2002-050A19 |
| 3 | AH0000-R050A1 | Outlet O-ring Set | ||
| 3 | AF0000-D05001 | Outlet Disperser Frit | ||
| 3 | AS2002-050A15 | Piston Frit Replacement Kit*, 5μm | ||
| 101mm i.d. | ||||
| With 2μm Frits | 2 | AS4004-101A18 | Piston Frit Replacement Kit*, 2μm | AS4004-101A20 |
| 2 | AS4004-101011 | Piston O-ring | ||
| 2 | AS4004-101012 | PTFE Sleeve | ||
| With 5μm Frits | 2 | AS4004-101A17 | Piston Frit Replacement Kit*, 5μm | AS4004-101A21 |
| 2 | AS4004-101011 | Piston O-ring | ||
| 2 | AS4004-101012 | PTFE Sleeve | ||
*柱头重装套装的零件见下表
弹簧柱的替换零件
除了上表中的重装套装，每个单独的替换零件可以从下表中订购。
SpringTM Column Replacement Parts
| 描述 | For 25mm i.d. | For 50mm i.d. | For 101mm i.d. |
| Top Restraint | --- | --- | AS4004-101008 |
| Bottom Restraint | --- | --- | AS4004-101009 |
| Piston Top | ---- | --- | AS4004-101010 |
| Piston O-ring | AS2002-025011 | AS2002-050011 | AS4004-101011 |
| Guide Tube | AS2002-025013 | AS2002-050013 | AS4004-101013 |
| Column Body, 40 cm Long Non-Water-Jacketed | FS2002-025040 | FS2002-050040 | AS4004-N10140 |
| Column Body, 70 cm Long Non-Water-Jacketed | FS2002-025070 | FS2002-050070 | AS4004-N10170 |
| Column Body, 40 cm Long Water-Jacketed | FS2002-W25040 | FS2002-W50040 | AS4004-101040 |
| Column Body, 70 cm Long Water-Jacketed | FS2002-W25070 | FS2002-W50070 | AS4004-101070 |
| Outlet Dispemer Frit | AF0000-D02501 | AF0000-D05001 | --- |
| Outlet Frit, 1μm | AF0000-C02501 | AF0000-B05001 | --- |
| Outlet Frit, 2μm | AF0000-C02502 | AF0000-B05002 | --- |
| Outlet Frit, 5μm | AF0000-C02503 | AF0000-B05003 | --- |
| Outlet O-ring Set | AH0000-R025A1 | AH0000-R050A1 | --- |
| Replacement Bolts, Nuts, and Washem for Non-Water-Jacketed Column | AS2002-025A08 | AS2002-050A10 | --- |
| Replacement Bolts, Nuts, and Washem for Water-Jacketed Column | AS2002-025A11 | AS2002-050A13 | --- |
| Threaded Spacer for 40cm Long Column | AS2002-025000 | AS2002-050000 | AS4004-101000 |
| Threaded Spacer for 70cm Long Column | AS2002-025001 | AS2002-050001 | AS4004-101001 |
| Threaded Flange | AS2002-025002 | AS2002-050002 | AS4004-101002 |
| Packing Spacer | AS2002-025003 | AS2002-050003 | AS4004-101003 |
| Unpacking Spacer for 40cm Long Columns | AS2002-025004 | AS2002-050004 | AS4004-101004 |
| Unpacking Spacer for 70cm Long Columns | AS2002-025005 | AS2002-050005 | AS4004-101005 |
| Piston | AS2002-025007 | AS2002-050007 | AS4004-101007 |
| Piston Frit Replacement Kit(Piston O-ring, 1μm Double Density Frit, PTFE Sleeve), 1μm | AS2002-025A12 | AS2002-050A14 | AS4004-101A16 |
| Piston Frit Replacement Kit(Piston O-ring, 2μm Double Density Frit, PTFE Sleeve), 2μm | AS2002-025A14 | AS2002-050A16 | AS4004-101A18 |
| Piston Frit Replacement Kit(Piston O-ring, 5μm Double Density Frit, PTFE Sleeve), 5μm | AS2002-025A13 | AS2002-050A15 | AS4004-101A17 |
| PTFE Sleeve | AS2002-025010 | AS2002-050010 | AS4004-101012 |
Dr.maisch收购格雷斯弹簧柱等相关产品
德国迈克（Dr.maisch）于2016年5月2号正式收购美国格雷斯（grace）公司旗下色谱柱以及 Spring弹簧柱/grace弹簧柱,SFC柱硬件以及相关的装柱机，收购的色谱柱品牌如下
Adsorbosphere,Allsphere, Brava, Econosphere, GraceSmart, Platinum, ProZap, Macrosphere, Prosphere and VisionHT® columns， MODcol® preparative chromatography brands, including Spring® columns, Multipacker® packing equipment, all associated hardware, replacement parts, and packing services，格雷斯公司从2016年5月份开始停止销售以上品牌的液相色谱柱和弹簧柱/装柱机等相关产品。上海宸乔生物科技有限公司做为Dr.maisch GmbH中国区的总代理，我们持续为客户提供原格雷斯的旗下的液相色谱柱和Spring弹簧柱，MODCol色谱柱，以及Multipacker装柱机，以及Dr.Maisch自有品牌的相关色谱柱，Dr.maisch GmbH公司代理商*书如下：
Spring弹簧柱/制备柱
