Column:

Sepax AAA, 4.6×250 mm

phase:

A: ACN : 0.1 M NaAc = 7 : 93 (v/v), pH=6.5; 
B: ACN : H₂O = 80 : 20 (v/v)

     Time (min): 
                B (%): 

0  15  18  25  30  30.01   45
0  15  24  40  40  100    100

Flow rate:

1.0 mL/min

Temperature:

36 ^oC

Detection:

UV 254nm

Injection Volume:

5 µL

Sample:

18 Amino acids

1.Asp

2.Glu

3.Ser

4.Gly

5.His

6.Arg

7.Thr

8.Ala

9.Pro

10.Tyr

11.Val

12.Met

13.Cys

14.Ile

15.Leu

16.Phe

17.Trp

18.Lys