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微培养箱 µ-Galaxy Cell Culture Incubator
面议KD Scientific Sying pump *
面议ibidi易必迪流体剪应力/剪切力实验系统 ibidi Pump System
面议ibidi气体孵育系统 Incubation Systems
面议ibidi气体孵育系统 Incubation Systems
面议ibidi易必迪活细胞维持系统,加热与孵育系统,活细胞长时培养观察
面议易必迪 ibidi 培养皿显微镜架/u-Dish Microscopy Rack
面议易必迪 ibidi 鲁尔接头 / Luer Connector Male
面议易必迪 ibidi嵌入式鲁尔注入端口/In-line Luer Injection Port
面议易必迪 ibidi 载玻片固定架(4片)/u-Slide Click Rack
面议易必迪 ibidi 载玻片显微镜架/ u-Slide Microscopy Rack
面议易必迪 ibidi 硅胶管/Silicone Tubing
面议货号
货号 | 描述 | 个/盒 | 价格 |
60641 | µ-Proteofection Kit VI, for direct transport of proteins through the cell membrane, 15 µ-Slides VI 0.4, ibiTreat, sterile, 250 µl PROTEOfectene®, 100 µg/ml R-Phycoerythrin in 100 µl PBS | 1 | ¥9810 |
60642 | µ-Proteofection Kit VI AB, for direct transport of antibodies through the cell membrane, 15 µ-Slides VI 0.4, ibiTreat, sterile, 250 µl PROTEOfectene® AB, 100 µg/ml FITC-IgG in 100 µl PBS | 1 | ¥9810 |
For the direct insertion of proteins (e.g., antibodies) into the cytosol of living cells
Cell culture, proteofection, and microscopy – all in one slide
Reagents optimized for proteins or antibodies, respectively
Fast and simple protocol
Applications:
Protein localization through fluorescent antibodies
Intracellular competitive protein inhibition
Protein studies in knock down organisms
Technical Features:
90 proteofection experiments per kit
PROTEOfectene® optimized for protein insertion
PROTEOfectene® AB optimized for antibody insertion
The kit contains PROTEOfectene® (AB), appropriate positive control reagents, and µ-Slide VI 0.4
Specifications / Content:
µ-Slide (ibidi) | µ-Slide VI 0.4, ibiTreat (15x) |
Reagent (Biontex) | PROTEOfectene® (250 µl) |
Positive control (Biontex) | R-Phycoerythrin, |
µ-Slide (ibidi) | µ-Slide VI 0.4, ibiTreat (15x) |
Reagent (Biontex) | PROTEOfectene®AB (250 µl) |
Positive control (Biontex) | FITC-IgG, 100 µg/ml |
The PROTEOfectene® reagents (Biontex Laboratories GmbH) were developed to enable the direct uptake of proteins and antibodies through the lipid membrane of cells by endocytosis. Using this procedure, shuttled proteins reach the cytosol and act directly in signal transduction. If the antibodies/proteins are labeled, their fate can be followed directly using microscopy.
To ensure that the proteofection procedure is working, either a fluorescent protein (R-Phycoerythrin) or an antibody (FITC-IgG) is included in the kit. These positive controls are useful tools for an easy evaluation of the experimental results.
Imaging of HeLa cells after proteofection with FITC-IgG (positive control included in the µ-Proteofection Kit VI)