易必迪 ibidi 细胞骨架 HT-1080 LifeAct-TagGFP2 Stable Cell

40101/40101易必迪 ibidi 细胞骨架 HT-1080 LifeAct-TagGFP2 Stable Cell

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2022-05-03 19:10:02
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易必迪 ibidi 细胞骨架 HT-1080 LifeAct-TagGFP2 Stable Cell Line
货  号:40101/40101

详细介绍

易必迪 ibidi 细胞骨架 HT-1080 LifeAct-TagGFP2 Stable Cell Line

货  号:40101/40101

A stable LifeAct-TagGFP2 expressing human fibrosarcoma cell line

Long-term actin visualization - 25 passages guaranteed

Characteristics proven to be identical to the parental HT-1080 cell line

LifeAct technology guarantees no interference with cytoskeletal dynamics

 

Applications:

Live cell imaging of cytoskeletal organization and dynamics

Functional cell-based assays, such as chemotaxis and migration

Usable as a characterized control in your experiments

To create the stable LifeAct-TagGFP2 expressing cell line, human fibrosarcoma cells were stably transfected with a plasmid coding for TagGFP2-labeled LifeAct .

The HT-1080 LifeAct-TagGFP2 cells present highly dynamic filamentous actin. As a result of stable LifeAct expression, perfect visualization of filamentous actin with very low background fluorescence is achieved without any interference to cellular dynamics. Moreover, full characterization of the cells revealed that they displayed identical behavior to wild type (wt) cells.

Due to the cell line’s fast growth and the absence of contact inhibition, it constitutes an ideal tumor model. These characteristics combined with bright and specific F-actin staining lead to a powerful tool with versatile applications in live cell imaging and high-content approaches.

 

Specifications:

Cells/vial

5 x 105

Medium

ibidi Freezing Medium

Storage

Liquid nitrogen

Biosafety Level

1

Sterility

Tested for yeast and bacteria 
including mycoplasma

Promoter

CAG (modified chicken β-actin)

Transgene

LifeAct-TagGFP2*

 

*For further information on TagGFP2, please refer to www.evrogen.com.


Origin information:

Organism

Homo sapiens (human)

Source

Connective tissue disease: fibrosarcoma

Age

35 years

Gender

Male

Ethnicity

Caucasian

Growth properties

Adherent

Morphology

Epithelial

 

Technical Features:

Homogeneous cell pool

Assured cell line identity

Purity tested

Optimal transgene expression for microscopy assays

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