品牌
生产厂家厂商性质
上海市所在地
微培养箱 µ-Galaxy Cell Culture Incubator
面议KD Scientific Sying pump *
面议ibidi易必迪流体剪应力/剪切力实验系统 ibidi Pump System
面议ibidi气体孵育系统 Incubation Systems
面议ibidi气体孵育系统 Incubation Systems
面议ibidi易必迪活细胞维持系统,加热与孵育系统,活细胞长时培养观察
面议易必迪 ibidi 培养皿显微镜架/u-Dish Microscopy Rack
面议易必迪 ibidi 鲁尔接头 / Luer Connector Male
面议易必迪 ibidi嵌入式鲁尔注入端口/In-line Luer Injection Port
面议易必迪 ibidi 载玻片固定架(4片)/u-Slide Click Rack
面议易必迪 ibidi 载玻片显微镜架/ u-Slide Microscopy Rack
面议易必迪 ibidi 硅胶管/Silicone Tubing
面议A support reagent for enhancing the efficiency of viral gene transfer into difficult to transduce mammalian and rodent cells
Efficient, due to 20- to 50-fold enhanced adenovirus transduction
A non-toxic, peptide-based solution
Gives access to nearly all cell types
Applications:
Adenovirus transduction
NOTE:
Please note that you will be working with samples containing an infectious virus. Follow the recommended NIH guidelines for all materials containing BSL–2 organisms.
Technical Features:
Gives access to cells that express little CAR (Coxsackie Adenovirus Receptor)
ibiBoost™ Adenovirus Transduction Enhancer has been specifically developed to improve the uptake of adenovirus vectors (e.g., rAV-LifeAct Adenoviral Vectors ) into cells and cell lines that are normally difficult to transduce. ibiBoost reagent is unique, because it is based on an adenovirus binding peptide. Due to this factor, ibiBoost changes the adenovirus surface in a way that the uptake of the adenovirus is improved for all permissive cell types, and it allows transduction of cell types previously not accessible to adenovirus transduction. Unlike chemical transduction enhancers, ibiBoost has no toxic effects. The use of ibiBoost is especially recommended if high ”Multiplicities of Infection“ (MOI), exceeding 500, have to be applied to a particular cell type.
Specifications:
Volume | 50 μl |
Amount | For 50 transductions à 2 × 107 IU |
Storage | 4°C |
ibiBoost™ Adeno Adenovirus Transduction
Enhancer significantly enhanced GFP
expression in rodent NIH-3T3 cells
Enhancing Transduction Efficiency
I: Genetic manipulation of target cells increases the CAR expression on the cell surface.
II: Genetic manipulation of the adenovirus binding domain addresses the non-CAR surface receptors of the target cell.
III: The ibiBoost Adeno Adenovirus Transduction Enhancer bridges the adenovirus surface to the cell membrane, thereby enhancing the gene expression by 20- to 50-fold into cell types that lack the primary adenovirus receptor CAR.