μ-Slide 4 well Ph+/80446

µ-Slide 4 well ^Ph+

                                                                                             Excellent Phase Contrast in the µ-Slide 4 well ^Ph+

An open µ-Slide with 4 wells and a special intermediate plate for excellent phase contrast and high-end fluorescence microscopy

·         Excellent phase contrast over the entire well – no meniscus

·         All-in-one chamber used for cell culture and microscopy

·         Observation of the sample through the coverslip-like bottom using high resolution microscopy

·         Fast and easy immunofluorescence, with no coverslips and no leakage

Applications:

·         C*tion and microscopy of cell cultures

·         Transfection assays

·         Fluorescence microscopy of both living and fixed cells

·         Live cell imaging over extended time periods

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Specifications:

Technical Features:

·         Open µ-Slide with 4 independent wells

·         Intermediate plate in each well to avoid meniscus formation

·         Easy filling, without air bubbles, through the lateral slot

·         Excellent optical quality for high-end microscopy

·         Compatible with staining and fixation

·         ibiTreat surface for optimal cell adhesion

·         Made of biocompatible plastic material – no glue and no leaking

Phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. When working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. In open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. This miscalibrates the phase rings, leading to poor contrast in the microscopic image.

µ-Slide 4 well                                                      µ-Slide 4 well ^Ph+

Working with the µ-Slide 4 well ^Ph+ diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.

The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.

Working with the µ-Slide 4 well ^Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.

Comparison Ph+ Well versus Standard Well: 
No Meniscus Effect in Ph+ Well

 µ-Slide 4 well ^Ph+
Excellent Phase Contrast
Excellent Fluorescence Microscopy